The experimental research of isolated limp perfusion on rabbit model
- VernacularTitle:肢体隔离性灌注的兔实验模型研究
- Author:
Yongli WANG
;
Linsun LI
;
Jie WANG
- Publication Type:Journal Article
- Keywords:
Isolated limp perfusion;
Model;
Rabbit
- From:
Journal of Interventional Radiology
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up an isolated limp perfusion (LIP) model of rabbit approximately asin the physiological state. Methods Measuring and calculating the average speed of blood circulation in rabbit's femoral artery with ultrasound, building up an isolated limp of perfusion system with a mini membrane oxygenator and a mini pump. Observing the values of blood analyses (pH, PO 2 and PCO 2) at prior , mid and post operation with differential exchanging speeds of oxygen, and probabilities of circulation failure with differential blood flow speeds, and the glucose change at several points of time and the difference between the groups with and without compensating glucose, and the dimensions of hind leg prior and post operation. Results Results of ultrasound showed that there was no difference in parameter between bilateral femoral arteries giving no effect on the results of differential groups. Total average flow speed was (12.0?1.8) ml/min. Blood analysis: the perfusion without oxygen exchange showed pH was lower than preperfusion and physiological low threshold. PO 2 was below the normal standard, PCO 2 was higher than prepefursion and physiological upper threshold. 100ml/min of oxygen exchange showed pH, PO 2 and PCO 2 were rearly normal post perfusion and 200ml/min group showed pH and PO 2 obviously higher than the normal physiological value. The gulcose was lower than the normal at 30min after perfusion, but could be recoveried under the compensation of glucose. There was significant difference between the groups processing with and without glucose. There was no circulation failure when the flow speed was in 10ml≤V
