Assay of The Contents of Ginsenoside Rg_1 and Rb_1, Notoginsenoside R_1 in Compound Danshen Dropping Pill by HPLC/ELSD
- VernacularTitle:HPLC-ELSD法测定复方丹参滴丸中人参皂苷R_(g1)、R_(b1)和三七皂苷R_1的含量
- Author:
Yongzhuo HUANG
;
Nisheng WANG
- Publication Type:Journal Article
- Keywords:
Compound Danshen Dropping Pill/Chemistry;
Ginsenoside/Analysis;
Notoginsenoside/Analysis;
Chromatography, High Pressure Liquid;
@ELSD
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for determining the contents of ginsenoside Rg1 and Rb1, notoginsenoside Rg1 in compound Danshen dropping pill by HPLC/ELSD. Methods: Ginsenoside Rg1 and Rb1, notoginsenoside R1 were pretreated with solid phase extraction(SPE). The extraction conditions were as follow: Hypersit NH2 column, acetonitrile-isopropanol-10mmol/L ammonium acetate( 15∶4∶1 ) as mobile phase (iced acetic acid adjusting pH as 5.0) and the flow rate at 0.6 mL/min. Results: The linear range was from 1.0 to 10.0?g for ginsenoside Rg1 and Rb1, and notoginsenoside Rg1. The average recovery was 95.3%～100.4%.The inter-day RSD and intra-day RSD were less than 2%and 4%respectively. Conclusion: The method is concise and accurate, and will be helpful for the quality control of Radix Notoginseng and its preparations.
