NEUROTROPHIC EFFECT OF ENSHEATHING CELLS ON GABAERGIC NEURONS in vitro
- VernacularTitle:嗅神经鞘细胞在体外培养时对GABA能神经元的作用
- Author:
Hao YANG
;
Zhe LIANG
;
Chunting WANG
;
Hualing CHENG
;
Gong JU
- Publication Type:Journal Article
- Keywords:
Olfactory ensheathing cell;
Neuron;
GABA;
Neurite;
Survival;
Mouse
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of the present study is to illustrate the effect of olfactory ensheathing cells(OECs) on the survival and neurite outgrowth of GABAergic neurons in vitro. Methods OECs were dissociated from olfactory bulb and neurons from spinal cord of E12 mouse. On the sixth day in vitro,the Millipore cultue blank with OECs was transferred to the neuron culture mediam and continue the co-culture for another 6 days.The cultured neurons were stained with anti-GABA antibody.The neurite of neurons was observed with an image system.The number of GABAergic positive neurons was counted under the microscope. Result The number of GABAergic neurons was 39^7?6^3 in co-culture groups,whereas the number of GABAergic neurons represented only 27^6?2^7 in control groups(CG),(P
