CHARACTERIZATION OF NEURAL STEM CELLS ISOLATED FROMHUMAN FETAL CEREBRAL CORTEX IN VITRO

VernacularTitle:人胚胎大脑皮层神经干细胞的分离培养
Author: Wei LI ; Kang WU ; Maolin QIN
Publication Type:Journal Article
Keywords: Neural stem cells; Cell culture; Human
From: Acta Anatomica Sinica 1955;0(03):-
CountryChina
Language:Chinese
Abstract: Objective To establish human neural stem cells(HNSCs) model for further basic research and clinical application. Methods Cells from human fetal cerebral cortex were collected and cultured with serum free midium and then identified for nestin immunocytochemical staning;The cells were induced to differentiate by 5% fetal bovin serum and identified by neurofilament-200(NF-200) and glial fibrillary acidic protein(GFAP) immunocytochemical staning. Results The harvested cells appeared as clusters in suspension and both NF-200 and GFAP positive cells were observed after induction.After 12 generations of culture,these cells retained the main characteristics of NSCs.Conclusions The HNSCs were harvested from human fetal cerebral cortex and this HNSCs model can be used for futher basic research and clinical applications.;