SIMULTANEOUSLY DEMONSTRATION OF TYROSINE-HYDROXYLASE(AND SOMATOSTATIN)GENE EXPRESSION AND PEPTIDE STORAGE IN SINGLE NEURON OF THE HEART
- VernacularTitle:同一心内神经元酪氨酸羟化酶、生长抑素的各自基因表达与贮存 原位杂交和/或免疫组织化学法光镜观察
- Author:
Guangqian LI
;
Huijun LI
;
Yuhu FAN
- Publication Type:Journal Article
- Keywords:
Tyrosine-hydroxylase;
Somatostatin;
In situ hybridization;
Immunohistochemistry;
Bufo bufo gargarizans
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To demonstrate simultaneously on transcription and translate levels the tyrosine-hydroxylase(and somatostatin)gene expression and peptide storage in same intrinsic cardiac ganglionic neuron of bufo bufo gargarizans. Methods Using immunohistochemical method,in situ hybrodization method and in situ hybrodization/immunohistochemical double labeling method on freeze-sections of sinus venanum cavanum of bufo bufo gargarizans atrial posterior wall (30*!?m)to observe THmRNA and /or TH-IR and SSmRNA and /or SS-IR dyeing neurons of intrinsic cardiac ganglia. Results There are kinds of positive neurons:1.TH-IR positive neurons;2.THmRNA positive neurons;3.TH mRNA/TH-IR double positive neurons and 1.SS-IR positive neurons;2.SSmRNA positive neurons;3.SSmRNA/SS-IR double positive neurons.Conclusion 1.TH mRNA and TH appearing in same intrinsic cardiac ganglionic neuron demonstrate that a part of intrinsic cardiac ganglionic neurons are possibly adrenergic sympathetic neurons;2.SSmRNA positive neurons and SSmRNA/SS-IR double positive neurons indicat that SS could be a neurotransmitter of intrincic cardiac ganglinic neurons.It provides neuroanatomy evidence of regulating cardiac function(excitatory conduct、cardiac muscle secrete and contract)by intrinsic cardiac SS-nergic neuron.
