SIMULTANEOUS DEMONSTRATION OF NEURONS AND NERVE FIBERS WITH SERIAL SECTIONING IN WHOLE CNS OF THE RAT
- VernacularTitle:同时显示大鼠中枢神经系统神经元和神经纤维的全程连续切片方法
- Author:
Deying CHEN
;
Jianjun LIU
- Publication Type:Journal Article
- Keywords:
Central nervous system;
Nerve fiber;
Neuron;
Cryostat serial sectioning;
Improved hematoxylin staining;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a practicable method of serial sections of whole CNS in the rat, in which both neurons and nerve fibers were showed at the same section. Methods After perfused fixation and quick freezing, the tissues of whole CNS were cut into serial sections with cryostat followed by hematoxylin stain. Results The nerve fibers were stained in blue color while the neurons were showed black-blue and the background was light-yellow.Conclusion Our method was feasible to show both neurons and nerve fibers at the same section with quick freezing, freezing serial sectioning and improved hematoxylin staining.
