CLONING OF FULL LENGH cDNA AND EXPRESSION OF RAT SMALL GTP-BINDING PROTEIN TC10 IN FACIAL NUCLEUS INDUCED BY INJURY
- VernacularTitle:大鼠面神经核小GTP结合蛋白TC10的表达和真核表达载体的构建
- Author:
Xin NIE
;
Yan JIN
;
Zhihong LI
;
Jian WANG
- Publication Type:Journal Article
- Keywords:
TC10;
Reverse transcrpit polymerase chain reaction;
Gene cloning;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of small GTP-binding protein TC10 during nerve injury and construct eukaryotic expression vector carrying rat TC10 cDNA. Methods Total RNA was extracted from rat facial nucleus, and the change of rat TC10 full-length cDNA was investigated by RT-PCR. PCR product was inserted into eukaryotic expression vector pcDNA3 to construct the pcDNA 3-TC10. The plasmid was identified by restriction enzyme analysis and sequencing analysis. Results After injury, the expression of TC10 rapidly increased, Restriction enzyme assay and sequencing assay showed that the construct we got was rat TC10 full-length cDNA construct.
