Angiotensin Ⅱ stimulates the synthesis of nucleic acid, protein and collagen in cultured hepatic stellate cells in rats
- VernacularTitle:血管紧张素Ⅱ刺激肝星状细胞核酸、蛋白质及胶原合成
- Author:
Yuqin WANG
;
Dingguo LI
;
Hongshan WEI
- Publication Type:Journal Article
- Keywords:
AngiotensinⅡ;
Hepatic stellate cell;
Proliferation;
Collagen synthesis
- From:
Chinese Journal of Digestion
1996;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different doses of angiotensin Ⅱ(Ang Ⅱ) on hepa tic stellate cells(HSC) proliferation and collagen synthesis in rats. Methods The pronase E and collagen Ⅰ were used to isolate HSC, 3H TdR, 3H Leu and 3H Pro incorporation methods were used to evaluate the effects of different doses of AngⅡ on HSC DNA, protein and proline synthesis. Results It showed that 10 -9 ～10 -6 mol/L AngⅡ could significantly increase the 3H TdR incorporation rate of HSC ( P
