Cloning, expression and activity of catalase gene of H.pylori
- VernacularTitle:幽门螺杆菌过氧化氢酶基因的克隆、高效表达及活性评价
- Author:
Yang BAI
;
Yali ZHANG
;
Jide WANG
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Catalase;
High expression
- From:
Chinese Journal of Digestion
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a highly expresses catalase of Helicobacter pylori (H.pylori) and to test it's activity. Methods The catalase DNA was amplified from H.pylori chromosomal DNA by PCR techniques, inserted into the prokaryotie expression vector pET 22b(+) and then transformed into the BL21(DE3) E.coli strain which expressed catalase recombinant protein. Then the activity of H.pylori catalase was assayed by the Beers and Sizers. Results DNA sequence analysis showed that the sequence of catalase DNA was the same as that published by GenBank. The catalase recombinant protein amounted to 24.4% of the total bacterial protein after inducing with IPTG for 3 hours at 37?C and the activity of H.pylori catalase was high in the BL21 (DE3) E.coli strain. Conclusions A clone of expressing high activity H.pylori catalase has been obtained, and thus a good foundation for further study has been laid.
