EFFECT OF TRANSPLANTATION OF THE NEURAL STEM CELLS MODIFIED WITH GENE OF NGF OR GDNF ON THE CHOLINERGIC NEURONS OF RASAL FOREBRAIN OF AD MODEL RAT
- VernacularTitle:NGF/GDNF基因修饰神经干细胞移植对AD模型鼠前脑胆碱能神经元的保护作用
- Author:
Yiwen RUAN
;
Qunfang YUAN
;
Chuanen WANG
;
Zhibin YAO
- Publication Type:Journal Article
- Keywords:
Neurotrophic factors;
Neural stem cell;
Gene therapy;
Acetylcholinesterase;
Neuron
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of a single or combined transplantation of the neural stem cells(NSCs) modified with gene of NGF or GDNF on the cholinergic neurons of basal forebrain of AD model rat. Methods The NSCs modified with gene of NGF or GDNF were implanted in single or combined into the lateral cerebral ventricle of the rats after fibria\|fornix transection.The rats were killed three weeks after transplantation and the brain sections concluding basal forebrain were cut coronally on a freezing microtome and were processed by immunohistochemistry staining with antibodies against ChAT.The numbers of ChAT positive neurons of medial septum(MS) and vertical diagonal band(VDB) were analyzied statistically with one way of Student\|Newman Kaels. Results In MS,the percentages of ChAT positive neurons at the lesion side to the intact side in NGF group was 81% which was significantly higher than that in the lesion group(34%),NSC group(36%) and GDNF group(50%), P 0\^05). Conclusion\ The injury cholinergic neurons can be protected in different extent after a single or combined transplantation of the neural stem cells modified with gene of NGF or GDNF.Among these three groups,greater protection was found in NGF group and NGF+GDNF group,and lesser protection in GDNF group.\;[
