The effect of hTR antisense PS-ODN in combination with cisplatin and adriamycin on human gastric cancer cell line
- VernacularTitle:硫代修饰端粒酶反义核酸联合顺铂、阿霉素对胃癌细胞作用的研究
- Author:
Xiaocang CAO
;
Naixia HUANG
;
Bangmao WANG
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
Telomerase;
Cisplatin;
Adriamycin
- From:
Chinese Journal of Digestion
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of hTR antisense PS-ODN on the susceptibility of human gastric cancer cell line SGC7901 to Adriamycin and Cisplatin. Methods hTR antisense PS-ODN with sequence CAG-TTAGGGTTAG which can recognize the RNA template of telomerase was transfected into SGC7901 by lipofectamine, and drug sensitivity test was performed with MTT method, telomerase activity was quantitatively measured by TRAP-PCR-ELISA methods, the apoptotic cells were measured with FCM. Results The telomerase activity in gastric cancer cell line SGC7901 after treatment with cisplatin and doxorubicin was different, and the change of telomerase activity of SGC7901 by cisplatin and doxorubicin was in time-dependent manner. The inhibitory ability of cisplatin and doxorubicin combined with hTR antisense PS-ODN in cell line SGC7901 was higher than that of these drugs used alone. Telomerase activity was dramatically declined during the cell incubation with hTR antisense PS-ODN combined with ADM or DDP. The hTR antisense PS-ODN increased susceptibility to cisplatin or doxorubicin-induced apoptotic cell death in gastric cancer cell line SGC7901. Conclusions The hTR antisense PS-ODN increased susceptibility to cisplatin or doxorubicin in gastric cancer cell line. This may be correlated with its inhibitory effects on telomerase activity and the ability of induced apoptotic cell death. These findings indicate that hTR antisense PS-ODN may represent a novel chemosensitive agent.
