Effect of intrauterine hepatitis B virus infection on hepatitis B vaccine inoculation in newborns
- VernacularTitle:子宫内乙型肝炎病毒感染对新生儿免疫接种的影响
- Author:
Jinlai MENG
;
Yafei YUE
;
Shulin ZHANG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Disease transmission vertical;
Leukocytes, mononuclear;
Vaccination;
Interleukin 2
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To study the effect and the mechanism of peripheral blood nuclear cells (PBMC) invaded by hepatitis B virus (HBV) on the artificial immunization in newborns Methods Fifty two newborns, whose mothers were hepatitis B surface antigen (HBsAg) positive, were immunized with hepatitis B immunoglobulin and hepatitis B vaccine (HBVac), and then followed for 7 months The newborns′ serum and PBMC HBV DNA was detected by nested PCR, hepatitis B surface antibody (HBsAb) was tested with solid phase radioimmunoassay PBMC from newborn were incubated with PHA and HBsAg The supernatant interleukin 2 (IL 2) level was mesured by enzyme linked immununosorbent assay (ELISA) Results The rate of vaccination failure was higher in the infants with PBMC HBV DNA positive than those with negative ( P
