Changes of ATPase in fetal rat′s cerebral mitochondria and endoplasmic reticulum subsequent intrauterus ischemia and hypoxia
- VernacularTitle:子宫内缺血缺氧及再灌注中胎鼠脑组织内质网与线粒体ATP酶的动态变化
- Author:
Weiwei SONG
;
Jianhua FU
;
Xianjing JIA
;
Yukun HAN
- Publication Type:Journal Article
- Keywords:
Fetal anoxia;
Cerebral ischemia;
Cerebral anoxia;
Ca(2+) Transporting ATPase;
Na(+) K(+) exchanging ATPase;
Mitochondria;
Endoplasmic reticulum
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of intracellular calcium and other ions disturbance by measuring the activity of Ca 2+ adenosine triphosphatase (Ca 2+ ATPase) and Na + K + adenosine triphosphatase (Na + K + ATPase) Methods Model of fetal rats ischemia and reperfusion was established The duration of ischemia was 15,30,45 and 60mins respectively;after ischemia for 15 mins, reperfusion for 1,4,8,15 and 24 hours There were 7 11 fetal rats sacrificed at different time points respectively, 12 rats in sham for control The mitochondria and endoplasmic reticulium (microsomia) were estracted and the activity of the enzyme was measured Results In the ischemia group: with the development of ischemia, the activity of Ca 2+ ATPase in mitochondria decreased gradually ( P
