Time-dependenct and dose-dependenct regulation of human progesterone receptor isoforms A and B in uterine endometrial carcimoma by human insulin-like growth factor-Ⅰ
- VernacularTitle:人类胰岛素样生长因子-Ⅰ调控子宫内膜癌细胞系孕激素受体亚型表达的研究
- Author:
Xiaohong ZHANG
;
Lihui WEI
;
Jianliu WANG
;
Zheng TU
- Publication Type:Journal Article
- Keywords:
Insulin like growth factor Ⅰ;
Receptors, progesterone;
Endometrial neoplasms;
Blotting, Western
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective We study the regulation of human progestrone receptor isoforms A and B in uterine endometrial carcinoma cell line by different concentration of human insulin like growth factor Ⅰ (IGF Ⅰ) for different time,to investigate the roles of IGF Ⅰ and progestrone receptor isoforms in uterine endometrial carcimoma Methods The uterine endometrial adenocarcinoma cell line HEC IB was cultured in vitro and the breast cancer cell line MCF 7 was used as control Western blot was applied to examine the changes of the two isoforms by different concerntration IGF Ⅰ for different time Results (1) In HEC IB cell line, 10 ng/ml IGF Ⅰ made hPRB up regulated in the first 24 h But according to lager concerntration and longer time, human progesterone receptor (hPR) B became down regulated, which were significant at 20 ng/ml IGF Ⅰ for 72 h and 40 ng/ml IGF Ⅰ for 48-72 h The change of hPRA was like hPRB (2) In MCF 7 cell line, 10 ng/ml and 40 ng/ml IGF Ⅰ made hPRA and hPRB significantly up regulated in 24, 48, 72 h Twenty ng/ml IGF Ⅰ made hPRB up regulated also in the first 24 h But in 48 h and 72 h, down regulation of hPRB was detected Twenty ng/ml IGF Ⅰ made hPRA down regulated in 24, 48, 72 h Conclusions (1) The regulation of IGF Ⅰ to hPR isoforms has cell type specific and dose dependenct and time dependenct (2) In HEC IB cell line, 10 ng/ml IGF Ⅰ made hPRB significantly up regulated in 24 h But following exposure to IGF Ⅰ at larger concentration and longer time, hPRB became down regulated The change of hPRA is like hPRB
