The comparison among extraction methods of exosomes from T cells of rats
10.3969/j.issn.1671-8348.2013.29.019
- VernacularTitle:大鼠T细胞源性exosome提取方法比较
- Author:
Zhen SUN
;
Chibing HUANG
;
Yajun SONG
;
Yirong CHEN
;
Chuangui LI
- Publication Type:Journal Article
- Keywords:
exosome;
ExoQuick Precipitation;
ultrafiltration and sucrose gradient centrifugation;
differential ultracentrif-ugation
- From:
Chongqing Medicine
2013;(29):3512-3514,3517
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purification methods of the exosomes derived form T cells were established in order to get high quan-tity exosomes .Methods Exosomes from T cells culture supernatants were purified by ExoQuick Precipitation ,ultrafiltration and sucrose gradient centrifugation ,differential ultracentrifugation ,and confirmed via using transmission electron microscopy .The pro-tein expression of the exosomes were analyzed by SDS-PAGE electrophoresis .Western blotting was used to test the expression of IL-2 .Results The protein concentration of the exosomes purified through ExoQuick Precipitation ,ultrafiltration and sucrose gradi-ent centrifugation were higher than through differential ultracentrifugation (P<0 .05) .SDS-PAGE displayed the difference among the exosome purified by three methods .Three kinds of exosomes all expressed IL-2 .Conclusion ExoQuick Precipitation ,ultrafiltra-tion and sucrose gradient centrifugation technique can obtain high purity and complete exosome sample .
