Effects of recombinant human leukemia inhibitor on the preimplantation mouse embryo development in vitro
- VernacularTitle:重组人白血病抑制因子对体外移植前鼠胚发育的影响
- Author:
Guoyun WANG
;
Xiaohui DENG
;
Huiqin ZHANG
;
Hongling YU
;
Sen JIANG
- Publication Type:Journal Article
- Keywords:
Lymphokines;
Growth inhibitors;
Fertilization in vitro;
Embryo transfer;
Fetal development
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effect of recombinant human leukemia inhibitory factor (rhLIF) on mouse embryo development in vitro Methods Mice were randomly divided to three groups, one in vivo control (group Ⅰ) and two in vitro (group Ⅱ and Ⅲ) Mice were sacrificed at 116 120 hours (group Ⅰ) and 44-48 hours (group Ⅱ and Ⅲ) subsequent human chorionic gonadotropin (hCG) injection Two cell embryos (group Ⅱ and Ⅲ) and blastocysts (group Ⅰ) were obtained Embryos in group Ⅱwere cocultured with human tubal fluid (HTF) + 10% human serum and in group Ⅲ with HTF + 10% human serum+rhLIF (1 000 U/ml) The number of embryo in different stage was recorded and compared Results Embryo in four, eight cell and morula was noted in group Ⅱ and Ⅲ, 87 7% versus 91 2% and 75 0% versus 85 4% respectively There was no significant difference. However, further embryo development to the blastocyst, expanded blastocyst, and hatching blastocyst in group Ⅱ (48 1%, 32 1% and 18 4%) was lower than that in group Ⅲ (82 3%, 59 7% and 36 3%) There was no difference between blastocyst in group Ⅰ and group Ⅲ (86 0% vs 82 3%). Conclusion RhLIF does not provide obvious stimulation in early mouse embryo, however, rhLIF can promote the growth, differentiation, and hatching of preimplantion blastocyst
