Experimental study on sulindac metabolites-induced apoptosis on human umbilical vein endothelial cell line ECV304
- VernacularTitle:舒林酸代谢产物诱导血管内皮细胞凋亡的实验研究
- Author:
Suying LIU
;
Youji FENG
- Publication Type:Journal Article
- Keywords:
Sulindac;
Endothelium, vascular;
Apoptosis
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sulindac metabolites on the proliferation and apoptosis of human umbilical vein endothelial cell line ECV304 in vitro Methods The proliferation of ECV304 was determined by methyl thiazolyl tetrazolium (MTT) method The cell cycle, apoptosis and the ultrastructure of ECV304 were detected by flow cytometry (FCM) and electron microscopy respectively Results MTT assay showed that sulfide inhibited the proliferation of ECV304, and the effects was dose dependent, the 50% inhibiting concentration (IC 50 ) was 200 ?mol/L FCM showed that sulfide changed cell cycle distribution, the cell cycle were: Go G 1 phase [control group (77 7?1 6)%, sulfone group (75 6?2 1)%, sulfide group (46 1?1 6)%] S phase [control group (13 6?1 2)%, sulfone group (16 4?2 3)%, sulfide group (27 3?2 1)%], G 2 M phase [control group (8 6?0 7)%, sulfone group (8 0?0 5)%, sulfide group (26 6?3 5)% ] The apoptosis rates in control group, sulfone group and sulfide group were (6 1?3 4)%, (4 8?2 1)% and (51 9?5 7)%, respectively Compared with the control group, sulfide can reduce the proportion of G 1 phase, increase the proportion of S phase and G 2 M phase significantly ( P
