Modulation of inducible nitric oxide synthase inhibitors on articular cartilage damage after intraarticular injection of interleukin-1? and lipopolysaccharides in rabbits
- VernacularTitle:一氧化氮合酶抑制剂调控白细胞介素1?和脂多糖对关节软骨的损害
- Author:
Jixing WANG
;
Wei SUN
;
Dadi JIN
- Publication Type:Journal Article
- Keywords:
Nitric oxide synthase;
Enzyme inhibitors;
Cartilage,articular;
Interleukin 1;
Lipopolysaccharides
- From:
Chinese Journal of Rheumatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of nitric oxide synthase inhibitors S methylisothiorurea (SMT) on cartilage metabolism after intraarticular injection of interleukin 1? (IL 1?) and lipopolysaccharides (LPS) in rabbits.Methods The experiments were performed in three groups:normal group,control group and SMT group.The effects of SMT on IL 1? and LPS induced iNOS mRNA expression ,NO production and NOS activity in synovium,synovial fluid and cartilage were detected after 8 h.Proteoglycan synthesis was measured by ex vivo incorporation of Na 2 35 SO 4 into cartilage after 48 h.Results IL 1? and LPS induced iNOS mRNA expression and increased NO release;SMT could reduce NO release and inhibit iNOS mRNA expression in synovium,synovial fluid and cartilage,and partly restore proteoglycan synthesis of cartilage.Conclusion iNOS inhibitor SMT can protect IL 1? and LPS induced cartilage damage in high concentration.
