Differential gene analysis and expression of GST-? mRNA in esophageal cancerous and noncancerous tissues
- VernacularTitle:食管癌组织和非癌组织中差异基因分析和GST-? mRNA的表达
- Author:
Feng XU
;
Liqun ZHAO
;
Mingzhou GUO
- Publication Type:Journal Article
- Keywords:
Esophageal cancer;
DNA fingerprinting;
Polymerase chain reaction;
GST-? mRNA
- From:
Chinese Journal of Digestion
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of GST-? mRNA in the development and pathogenesis of esophageal cancer. Methods Twenty two matched pairs of esophageal cancerous and noncancerous tissues were obtained from 22 patients with esophageal cancer in an endemic region. AP-PCR was used for determining the differential gene fragments and RT-PCR for detecting the expression of GST-? mRNA. Results Differential random amplified fragments were found in 6 cancerous tissues, and none of the noncancerous tissues. The 5T differential gene fragments were of 1.0 kb, and by cloning, sequencing, and sequence homology analysis, no homologous sequence was found in the gene library. The expression rates of GST-? mRNA in cancerous and noncancerous tissues were 54.5%(12/22) and 18.2%(4/22), respectively. Conclusions Whether the 5T differential gene fragment is a new gene candidate or is a marker of oncogene remains to be further studied. The expression of GST-? mRNA in esophageal cancerous tissue was markedly enhanced.
