Effects of fed-batch carbohydrate, nitrogen, phosphorus on growth of Taxus chinensis var. mairei cell during suspension culture
- VernacularTitle:悬浮培养过程中碳、氮、磷的补加对南方红豆杉细胞生长影响的研究
- Author:
Ping HU
;
Yingjin YUAN
;
Zhiqi MIAO
- Publication Type:Journal Article
- Keywords:
Taxus chinensis var. mairei (Lemee et L?vl.) Cheng et L. K. Fu;
suspension culture;
growth;
carbohydrate
- From:
Chinese Traditional and Herbal Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To solve the problem that Taxus chinensis var. mairei (Lemee et L?vl.) Cheng et L. K.Fu grows slowly. Methods The consumption of phosphorus during the cell suspension culture and the effect of fed-batch carbohydrate, nitrogen and phosphorus on cell growth and living-cell activity were assayed. Results The carbohydrate was exhausted in the middle phase of suspension culture, dificiency of carbohydrate led to inhibition of cell growth in the late culture. Conclusion Compared with the control group, the cell growth rate and the cell density in the fed-batch carbohydrate group were increased significantly, and the cell growth rate was up to 83%.
