Expression of HOXA10 gene in human endometrium and its relationship with unexplained infertility
- VernacularTitle:HOXA10基因在子宫内膜组织中的表达及与不孕的关系
- Author:
Hong LI
;
Shiling CHEN
;
Fuqi XING
- Publication Type:Journal Article
- Keywords:
Gene;
Endometrium;
Ovum implantation;
Infertility, female
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of HOXA10 gene in the endometrium of normal fertile women and patients with unexplained infertility during different phases of menstrual cycle. Methods Endometrium samples were obtained by curettage in 52 normal fertile women and 38 patients with unexplained infertility during different phases of menstrual cycle, HOXA10 mRNA expression were detected by in situ hybridization and reverse polymerase chain reaction (RT-PCR). Results (1) HOXA10 mRNA were detected in the glandular and stromal cells of endometrium of fertile women during the menstrual cycle. By in situ hybridization (positive unite, PU), HOXA10 mRNA levels were significantly higher in the mid-secretory phase [glandular cells (G) 5.69?0.57,stromal cells (S) 7.48?0.67] and late-secretory phase(G 5.99?0.40,S 7.98?1.08) than those in late proliferative phase (G 3.35?0.20,S 3.20?0.37) and early secretory phase (G 3.07?0.26,S 3.18?0.27)(P
