Establishment and biological characteristics of nude mice xenograft tumor models of human endometrial carcinoma
- VernacularTitle:人子宫内膜癌裸鼠模型的建立及其生物学特性的研究
- Author:
Xiugui SHENG
;
Jianheng SUN
;
Chunxiao ZHOU
;
Shuzhen WANG
- Publication Type:Journal Article
- Keywords:
Endometrial neoplasms;
Neoplasm transplantation;
Disease models, animal;
Immunohistochemistry
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish nude mice xenograft tumor models of human endometrial carcinoma for basic and clinical study. Methods Fourteen samples of human endometrial carcinoma were subcutaneously heterotransplanted to nude mice (BALB/C, nu/nu),3～4 mice were transplanted for every sample. Results The initial take rate was 42.8%,then it increased to 100.0% after the 5th passage. Five of 14 samples were transplanted for 48～63 passages.The characteristics of histology,ultrastructure and chromosome were identical to those of human donour tumors. The DNA ploidy was consistent for SL-1,SL-2 and SL-3,but changed for SL-4 and SL-5 by cytometric analysis. The expression of estrogen receptor and progesterone receptor for 5 models was all negative. The expression of p53 was positive for SL-1,SL-2 and SL-4,but negative for SL-3 and SL-5.The expression of c-erbB-2 was positive for SL-1,SL-2 and SL-4,negative for SL-3 and SL-5.The expression of p16 was positive for SL-2 and SL-3,negative for SL-1,SL-4 and SL-5. Conclusions Five nude mice xenograft tumor models of human endometrial carcinoma were established successfully. It will be helpful for the basic and clinical study of human endometrial carcinoma.
