Comparison of Trimethoprim Content in Compound Dihydroartemisinin Tablets Determined by Dual-wavelength Spectrophotometry and HPLC
- VernacularTitle:HPLC和双波长分光光度法测定复方双氢青蒿素片中甲氧苄啶含量的比较
- Author:
Wenzhong XIAO
;
Meiyi ZHANG
;
Yanfang LIN
;
Lizhi ZHAN
;
Linchun FU
;
Guoqiao LI
- Publication Type:Journal Article
- Keywords:
COMPOUND DIHYDROARTEMISININ TABLETS/standard;
TRIMETHOPRIM/analysis;
CHROMATOGRAPHY,HIGH PRESSURE LIQUID;
DUAL WAVELENGTH SPECTROPHOTOMETRY
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of dual-wavelength spectrophotometry and HPLC on the content of trimethoprim(TMP)in Compound Dihydroartemisinin Tablets.Methods HPLC was performed in a column of C 18 with acetonitrile and 0.75% diethylamine(15∶85,adjusting pH to 2.5 by phosphoric acid)as the mobile phase and the detecting wavelength was at 271nm.The detecting wavelength was also at 271nm with reference wavelength at 366nm in dual-wavelength spectrophotomerty.Results Within 20.0～100.0 ?g/mL,TMP has a good linearity(r=0.999 94)by HPLC,and the average recovery was 100.9% with RSD being0.24%(n=5).By dual-wavelength spectrophotometry,a good linearity(r=0.999 95)of TMP was within 5.0～25.0 ?g/mL,and the average recovery was 100.0% with RSD being 0.45%(n=5).Conclusion Both dual-wavelength spectrophotometry and HPLC can be used to determine the content of TMP in Compound Dihydroartemisinin Tablets,but the former can detect the content of TMP directly without the disturbance of piperaquine phosphate and is simple,rapid and accurate.
