Quantitative determination of spinosin in seed of Ziziphus jujuba var. spinosa by RP-HPLC
- VernacularTitle:RP-HPLC法测定酸枣仁中黄酮碳苷的含量
- Author:
Yujuan LI
;
Ping LI
;
Huijun LI
;
Kaishu BI
- Publication Type:Journal Article
- Keywords:
the seed of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou;
spinosin;
RP-HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop a new method for the quantitative determination of spinosin in the seed of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou Methods The determination was carried on RP-HPLC, using Hypersil-C 18 column, acetonitrile∶water (17∶83, v/v) as mobile phase with a flow rate of 0 8 mL/min and detected at the wave length of 334 nm Results The calibration curve was linear in the range of 5 280～ 14 08 ?g/mL The average recovery rate was 95 8% Conclusion The method is appropriate for the quantitative determination of spinosin
