Synthesis and identification of methylparaoxon artificial antigen
10.3969/j.issn.1000-8861.2001.02.021
- VernacularTitle:甲基对氧磷人工抗原的合成及鉴定
- Author:
Weiguo ZHANG
;
Zhaoyang MA
- Publication Type:Journal Article
- From:
Immunological Journal
2001;(2):144-145,154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To synthesize the artificial antigen methylparaoxon （M1600）.Methods Methylparaoxon was reduced into amino-methylparaoxon by using acetic acid-zinc power-hydrochloric acid. Artificial antigens M1600-BSA， M1600-TTH were synthesized by conjugating amino-methylparaoxon to bovine serum albumin （BSA）and tachypleus tridentatus hemocyanin （TTH）directly after diazotization.Results Rabbits had been immunized with M1600-BSA for 10 weeks， and the high titer and high specificity antiserum from those rabbits was testified by doubled agar gel diffusion and indirect ELISA．Conclusion An artificial antigen was obtained successfully and this made it possible to establish the immunoassay of M1600.
