Simultaneous Determination of Sanguinarine and Chelerythrine in Macleaya Microcarpa(Maxim) Fedde by RP-HPLC
- VernacularTitle:RP-HPLC法同时测定小果博落回中血根碱和白屈菜红碱的含量
- Author:
Caixian AN
;
Guangde YANG
;
Jiantao YE
;
Junmei FU
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
macleaya microcarpa(Maxim) fedde;
sanguinarine;
chelerythrine
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the content of sanguinarine and chelerythrine in Macleaya microcarpa(Maxim) Fedde. Methods: RP-HPLC was adopted, kromasil C 18 column (150mm?4.6mm.5?m) with a mobile phase acetonitrile-0.1%(V/V) phosphoric acid solution(25∶75) by UV detector at 270nm. Results: The average recoveries of chelerythrine and sanguinarine were 90.2%,92.8%,RSD were 1.5%,2.4% respectively. Conclusion: A simple, rapid and sensitive method with good precision was established.
