Association between refluxate in esophagus and expression of p53, Cyclin D_1, p21 and p16 in esophageal mucosa
- VernacularTitle:食管内反流物成分与p53、细胞周期素D_1、p21、p16表达的相关性研究
- Author:
Wen WANG
;
Guoming XU
;
Zhaoshen LI
- Publication Type:Journal Article
- Keywords:
Reflux;
Rat;
p53;
Cyclin D_1;
p16;
p21
- From:
Chinese Journal of Digestion
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of gastroesophageal reflux and duodenoesophageal reflux on expression of p53, Cyclin D 1, p21 and p16 in esophageal epithelium in rats. Methods Rat models of gastroesophageal reflux (G), duodenogastroesophageal reflux (DG), duodenoesophageal reflux (D) and control group (C) were set up by operations. All esophagi were draw at 1,2,4 week after operation and the expressions of p53, Cyclin D 1, p21 and p16 protein were measured by immunohistochemistry analyses. Results The levels of p53, Cyclin D 1 protein expression in reflux groups were significantly greater than those in C group, and those in D and DG group were significantly higher than in G group. The expressions of p16 protein were similar in each group. The number of p21 positive cells in D and DG group was fewer than C group at 4 week, and the staining pattern correlated well with p53 staining. Conclusions Both gastric juice and duodenal contents can change expression of p53, Cyclin D 1, p21 protein in esophageal epithelium, but not that of p16. The effects of duodenal contents is stronger than gastric juice. The changes of above genes expression may associate with the pathogenesis of reflux esophagitis and its complications.
