Effect of Ovariectomized Rat Serum Containing Bushen Yijing Decoction on the Absorption Function of Osteoclast
- VernacularTitle:含补肾益精方的切卵大鼠血清对破骨细胞骨吸收功能的影响
- Author:
Hongsheng ZHAN
;
Yinyu SHI
;
Yongfang ZHAO
- Publication Type:Journal Article
- Keywords:
Bushen Yijing Decoction/pharmacology;
Osteoporosis/TCD therapy;
@Serologic pharmacology
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of ovariectomized rat serum containing Bushen Yijing decoction (BYD) on the absorption function of osteoclast. Method: Fourty 10 - month - old female SD rats were randomly allocated to four groups, among which 30 rats were subjected to ovariectomy to establish the osteoporosis model, and 10 rats only received mimic operation. After 3 months, the model rats were given saline, BYD and estrogen respectively. Three months later, all the rats were killed to obtain the sera. Osteoclasts were isolated from the extremities of 1 - day SD rats and incultured on the ivory slices. The lacunar number formed after bone absorption was observed. Results: Compared with the mimic operation group, the number of lacurae of ovariectomized groups increased by 74. 1 % ,while that of the BYD group and estrogen group decreased by 65.7% and 59.3% respectively, showing significant differences with the mimic group (P
