Cloning of the genes associated with the pathogenesis of pregnancy induced hypertension using subtractive hybridization
- VernacularTitle:应用改良消减杂交技术克隆妊娠高血压综合征致病相关基因
- Author:
Yuanqing YAO
;
Juanzi SHI
;
Wei YAN
- Publication Type:Journal Article
- Keywords:
Pregnancy complications, cardiovascular;
Hypertension;
Hybridization;
Polymerase chain reaction;
Cloning, molecular
- From:
Chinese Journal of Obstetrics and Gynecology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone the genes related to pregnancy induced hypertension (PIH) for the study of PIH pathogenesis. Methods The differential expressed cDNA of PIH and normal pregnant placenta were cloned using modified polymerase chain reaction based subtractive hybridization. The differential cDNA were sequenced. Results Ammong 86 clones from subtractive hybridization, 14 clones containing differential expressed gene fragments in PIH and normal placenta were identified, and these might be the genes associated with PIH. Compared with the expressed sequence tags (EST) in dbEST of National Center for Biotechnology Information, it showed that 11 of them were known gene fragments, and three were unknown. The three unknown gene fragments were registered in GenBank, the accession number is AF 232216, AF 232217, AF 233648 respectively. Conclusions Fourteen PIH related genes were cloned with subtractive hybridization. A new approach to study on the pathogenesis of PIH is presented.
