The inhibitory effect of oxymatrine on hepatitis B virus in vitro
- VernacularTitle:氧化苦参碱抗乙型肝炎病毒的体外实验研究
- Author:
Jiqiang LI
;
Yingxuan CHEN
;
Minde ZENG
- Publication Type:Journal Article
- Keywords:
Oxymatrine;
Anti HBV;
HepG2 2.2.15
- From:
Chinese Journal of Digestion
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effect of oxymatrine on hepatitis B virus(HBV) in vitro. Methods Microparticle enzyme immunoassay, bDNA signal amplification assay was used for determining secrected HBsAg/HBeAg in the culture medium, HBV DNA from core particles in the cytoplasm and MTT colorimetric assay was used to assay the oxymatrine cytotoxity. Results The inhibitory rates of HBsAg and HBeAg were 40.57% and 48.27% by oxymatrine at the concentration of 2 000 ?g/ml. At 100～2 000 ?g/ml, it can remarkably decrease the level of viral core associated HBV DNA in the cytoplasm. No significant toxicity was shown in such concentrations. Conclusion Oxymatrine has a potential anti HBV activity in vitro.
