Determination of matrine in Sophora subprostrala Chun et T.Chen by HPLC
- VernacularTitle:HPLC测定山豆根中苦参碱的含量
- Author:
Fuliang DENG
;
Benmei CHEN
;
Shaoxian LIANG
;
Guohua CHEN
;
Lingwei XIA
- Publication Type:Journal Article
- Keywords:
matrine;
Sophora subprostrala Chun et T. Chen;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the method for determining matrine in Sophora subprostrala Chun et T.Chen. by HPLC. Methods: A CLC-phenyl column was selected as separation column at 30 ?C . Matrine was isocratically eluted by methanol-water-triethylamine (50∶50∶0.05,v/v) at flow rate of 1.0ml?min -1 . The peak of matrine was monitored at UV 219nm. Results: The detection limit was 2ng?ml -1 . The recovery of the added sample was above 95%. RSD of intra-day and inter-day was less than 2% and 4%, respectively. The linear range was 1.0～1000?g?ml -1 . Conclusion: The method was simple, rapid and producible.
