Determination of Astragloside IV in Extraction of Radix Astragali by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定黄芪提取物中黄芪甲苷的含量
- Author:
Yongping WU
;
Yongmei XU
;
Yuan CAO
;
Zhengzhong CAO
- Publication Type:Journal Article
- Keywords:
HPLC-ELSD;
extractive of Radix Astragali;
Astragaloside IV;
spray-dry
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a method to determine Astragaloside IV in spray-dry of Radix Astragali by HPLC-ELSD. Methods: ELSD was used to determine directly Astragaloside IV in spray-dry of Radix Astragali on Elite-OSD column, using CH 3CN-H 2O(36∶64) as a mobile phase. The flow rate was 1.0 ml/min. The temperature of drift tube for ELSD was 105 ?C and the flow rate of N 2 was 2.84 ml/min. Results: The recovery of the added sample was 91.68% and RSD 1.64%. Soluble amylum was helpful for spray-dry but it can influence was determination of Astragaloside IV. Conclusion: The method is accurate and can be used in the determination of Astragaloside IV in spray-dry of Radix Astragali. Better adjuvant should be found.
