Lack of Mutagenicity Potential of Periploca sepium Bge. in Bacterial Reverse Mutation (Ames) Test, Chromosomal Aberration and Micronucleus Test in Mice.
- Author:
Mei Shu ZHANG
1
;
In Seok BANG
;
Cheol Beom PARK
Author Information
1. Biotoxtech. Co. Ltd., Ochang, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Reverse mutation;
Chromosome aberration;
Micronucleus;
Mice;
Periploca sepium Bge
- MeSH:
Animals;
Arthritis, Rheumatoid;
Biotransformation;
Bone Marrow;
Chromosome Aberrations;
Cricetinae;
Cricetulus;
Erythrocytes;
Escherichia coli;
Glycosides;
Lung;
Medicine, Chinese Traditional;
Mice;
Micronucleus Tests;
Periploca;
Salmonella typhimurium
- From:Environmental Health and Toxicology
2012;27(1):e2012014-
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: The root barks of Periploca sepium Bge. (P. sepium) has been used in traditional Chinese medicine for healing wounds and treating rheumatoid arthritis. However, toxicity in high-doses was often diagnosed by the presence of many glycosides. The potential mutagenicity of P. sepium was investigated both in vitro and in vivo. METHODS: This was examined by the bacterial reverse mutation (Ames) test using Escherichia coli WP2uvrA and Salmonella typhimurium strains, such as TA98, TA100, TA1535, and TA1537. Chromosomal aberrations were investigated using Chinese hamster lung cells, and the micronucleus test using mice. RESULTS: P. sepium did not induce mutagenicity in the bacterial test or chromosomal aberrations in Chinese hamster lung cells, although metabolic activation and micronucleated polychromatic erythrocytes were seen in the mice bone marrow cells. CONCLUSIONS: Considering these results, it is suggested that P. sepium does not have mutagenic potential under the conditions examined in each study.