Experimental study on resistance to cisplatin and taxol in human ovarian cancer SKOV3ip1 multicellular aggregates
- VernacularTitle:顺铂和紫杉醇对人卵巢浆液性囊腺癌细胞系SKOV3ip1多细胞团簇的耐药性实验研究
- Author:
Jianli CHEN
;
Youji FENG
;
Xianrong ZHOU
- Publication Type:Journal Article
- Keywords:
Drug resistance, neoplasm;
Ovarian neoplasms;
Cystadenocarcinoma, serous;
Cisplatin;
Paclitaxel
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the chemosensitivity of ovarian cancer SKOV3ip1 multicellular aggregates to cisplatin and taxol and to explore the possible mechanisms accounting for the effect Methods Liquid overlay system was employed to obtain multicellular aggregates (MCA) We detected the resistance with trypan blue exclusion testing, clonogenic assay, cell cycle profiles and apoptosis with flow cytometry Results MCA cells showed higher cell viability than monolayer cells ( P =0 045 and P =0 003, respectively). After 40 ?mol/L cisplatin exposure for 12 hours, no clone (≥50 cells) was formed After 10 ?mol/L taxol exposure for 12 hours, the clone formation showed significant difference in 100 cell group between multicellular aggregates and monolayer cells ( P
