DNA-associated autoantibodies to cell membrane for rapid detection of systemic lupus erythematosus
- VernacularTitle:抗细胞膜DNA自身抗体快速检测系统性红斑狼疮
- Author:
Shi CHEN
;
Rulin JIA
;
Lei HAN
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
Autoantibodies;
Antibodies,antinuclear;
Fluoroimmunoassay
- From:
Chinese Journal of Rheumatology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the expression of autoantibodies to cell membrane associated with DNA (mDNA) in systemic lupus erythematosus (SLE).The specificity of these antibodies differs from antibodies to double stranded DNA.Method Using indirect immunofluorescence,serum samples were detected giving a characteristic pattern of peripheral membrane fluorescence on cultured HL60.Results This pattern was observed in 81 of 90 serum samplas of SLE patients,but absent in the serum samples of 35 rheumatoid arthritis,31 ankylosing spondylarthritis and 42 blood donors.In 20 Sjgren syndrome′s patients only one showed a positive test.Conclusion This novel rapid immunofluorescence method may serve as an identification test of SLE patients.Due to the sensitivity (90%) and specificity (98 8%),it is better than the other diagnostic tests such as the detection of antibodies to dsDNA and Sm.
