Study on the high performance liquid chromatography with fluorescence detection of gastric juice in the diagnosis of gastric carcinoma
- VernacularTitle:胃液高效液相荧光光谱对胃癌诊断的价值
- Author:
Huilan GUO
;
Liya ZHOU
;
Sanren LIN
- Publication Type:Journal Article
- Keywords:
Gastric carcinoma;
Gastric juice;
High performance liquid chromatography;
Fluorescence spectrum
- From:
Chinese Journal of Digestion
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the value of the high performance liquid chromatography (HPLC) with fluorescence detection of gastric juice in the diagnosis of gastric carcinoma. Methods After being super centrifuged, the supernatant of the gastric juice was chromatographed by reversed phase HPLC using C 18 column as the stationary phase and 20% methanol/H 2O as the mobile phase. The flow rate was set to 1 ml/min. The eluate was then detected with a fluorometer. Results 251 patients with different gastric diseases including 40 cases with gastric carcinoma were enrolled in our study. There were two characteristic peaks in the chromatograms, which appeared in both the benign and malignant lesions. The difference between benignancy and malignancy was the areas of the two peaks. The areas in the malignancy were much larger than those of the benignancy ( P
