Effect of antioxidants on lysis of red blood cells induced by saponins
- VernacularTitle:抗氧化剂对皂苷溶血作用的影响
- Author:
Hongyi WANG
;
Boyang YU
;
Luyong ZHANG
;
Min QIAN
- Publication Type:Journal Article
- Keywords:
saponin;
hemolysis;
antioxidants
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To study the hemolytic effect of total saponins from Ginseng (Gs), Radix Ophiopogonis (ROs) and Shengmaisan, a compound preparation of the two saponins, and the antihemolytic effect of antioxidants on hemolysis. Methods The percentage of hemolysis was indirectly calculated by measured cyanomethemoglobin, and morphological changes of red blood cells (RBCs) was examined by light microscope. Results Gs did not lead to hemolysis while both ROs and Shengmaisan induced lysis in a concentration dependent manner. RBCs deformation can be observed by light microscopic study. The hemolysis was partly inhibited by addition of glucose or antioxidants including mannitol and ?-tocopherol. Conclusion The intensity of hemolytic effect varied with different saponins present in the compound preparation, and may be associated with the type of saponin and partly due to its ability to enhance lipid preoxidation of RBCs membrane.
