OVERGROWTH OF EYE LENSES VIA INHIBITION OF GAP JUNCTION FORMATION AND ITS RELATION WITH LENS PROTEIN PHOSPHORYLATION IN CHICKEN EMBRYOS
- VernacularTitle:抑制缝隙连接形成引起鸡胚晶状体过度生长与晶状体蛋白磷酸化的相关性(英文)
- Author:
Haiying LI
;
Zhonghao XIA
;
Yu ZHAO
;
Xinren LI
;
Wenna LIN
- Publication Type:Journal Article
- Keywords:
Gap junction;
Major intrinsic protein(MIP);
Monoclonal antibody;
Growth control;
Protein phosphorylation;
Lens
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to further study the role of gap junction(GJ) in growth control; and explore the possible mechanisms for the inhibition of GJ formation. Method A monoclonal antibody,called ND6, which is specific for MIP(major intrinsic protein)in the plasma membrane of eye lens fiber cells in chicken,was injected into the right eyes of chicken embryos at development stage 20, while the left eyes were not injected and served as controls. The size of the lenses was measured 24 hours after the ND6 treatment; the protein phosphorylation of the lenses, including intact and homogenized lenses was also analyzed 24 hours after the ND6 treatment. Results The size of the lenses from treated eyes was significantly larger than those of the control ones( P
