APOPTOSIS OF HEPATOMA BEL 7402 CELL LINE INDUCED BY ALLICIN
- VernacularTitle:大蒜素诱导人肝癌BEL-7402细胞凋亡
- Author:
Honglian SHAO
;
Hua XIN
;
Yumei ZHAI
;
Xuan GAO
;
Qian WANG
;
Haiqing GAO
- Publication Type:Journal Article
- Keywords:
Allicin;
Hepatocellular;
Apoptosis
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective\ To investigate apoptosis of Hepatoma BEL\|7402 cells induced by allicin. Methods\ The inhibit effect of allicin on BEL\|7402 cells was investigated with MTT assay. Apoptosis was ascertained by cell morphology under light microscope, electron microscope and flow cytometry. Results\ BEL\|7402 cells were suppressed with dose and time dependent relationship after exposed to allicin of 10,20,40 mg/L for vairous lengths of time. Exposed to allicin at 60 mg/L for 3 hours, BEL\|7402 cells showed typical morphology characters of apoptosis: the microvilli of the cell surface disappeared, the cell shrink and decreased in volume and blebs or ball\|like bodies appeared. Typical nuclear condensation, margination and fragmentation were observed. Apoptotic cells were of trypan blue\|rejected staining. Percentage of apoptotic cells of control group and inductive group was 2\^02?0\^37%,78\^48?3\^15% respectively by trypan blue, and 1\^78?0\^48%, 74\^07?3\^94% by flow cytometry. Cells in G\-0/G\-1, S, G\-2/M phases of control group before induction were 47\^66?2\^72%, 22\^06?2\^04%, 30\^25?3\^78% respectively.Each of them is lower than the percentage of apoptotic cells. Conclusion\ Allicin induced apoptosis of hepatoma BEL\|7402 cells.Apoptotic cells were supposed to be initiated in some phases of cell cycle.\;[
