Effects of Blood-stasis-syndrome Rabbit Serum on Gene Expression of Activating Factors in Cultured Vascular Endothelial Cells
- VernacularTitle:血瘀证兔血清对培养的血管内皮细胞活性因子基因表达的影响
- Author:
Yunbo CHEN
;
Mengjun HOU
;
Qi WANG
;
Weixiong LIANG
;
Zehuai WEN
;
Shilon LAI
- Publication Type:Journal Article
- Keywords:
BLOOD STASIS/pathophysiology;
ENDOTHELIUM, V ASCULAR;
CELL CULTURE;
ENDOTHELIN;
NITRIC OXIDE SYNTHASE;
GENE EXPRESSION;
DISEASE MODELS, ANIMAL;
RABBITS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanism of blood_stasis syndrome (BSS) by studying the changes of gene expression of endothelin (ET) and constit utive nitric oxide synthase (cNOS) in vascular endothelial cells (VEC) cultured w ith BSS rabbit serum. Methods ET_1 mRNA expression and cNOS mRNA expression were analyzed by semi_quantitative reverse transcription and polymerase chain re action method. Results ET_1 mRNA expression level was increased and cNOS mRN A expression level was decreased in VEC cultured with BSS rabbit serum (Group A) as compared with VEC cultured with normal rabbit serum (Group B) or without rab bit serum (Group C) (P
