Effects of Xuefu Zhuyu Decoction on the Morphological Features of Vascular Endothelial Cells Injured by Serum of Blood-stasis-syndrome Rabbits
- VernacularTitle:血府逐瘀汤对用血瘀证兔血清损伤的血管内皮细胞的形态学影响
- Author:
Qi WANG
;
Yunbo CHEN
;
Shilong LAI
;
Weixiong LIANG
;
Zehuai WEN
- Publication Type:Journal Article
- Keywords:
BLOOD STASIS/pathophysiology;
ENDOTHELIUM, VASCULAR;
CELL CULTURE;
XUEFU ZHUYU DECOCTION/ther.use;
DISEASE MODELS, ANIMAL;
RABBITS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of Xuefu Zhuyu Decoction (XZD) on vascular endothelial cells (VEC) injured by the serum of blood_stasis_syndrome ( BSS) rabbits. Methods The morphological features of VEC cultured with norma l rabbit serum (Group A), BSS rabbit serum(Group B), the serum of normal rabbit medicated with XZD(Group C) and the serum of BSS rabbit medicated with XZD(Group D) were observed respectively under light microscope and electron microscope. Results Under light microscope, VEC was shrinked, intercellular space widene d and cytoplasm contained dark granules in Group B. The change of intercellular space was not obvious in Group D. Under electron microscope, pinocytotic vesicle s increased, rough endoplasmic reticulum (RER) decreased and dilated, mitochondr ia became smaller and indistinct and only a small amount of microvilli appeared on cell membrane with top expanded and merged in VEC of Group B as compared with Group A and Group C. Less RER but no dilatation and fewer microvilli without to p merged were found in VEC of Group B. Conclusion The serum of BSS rabbits c an injury normal VEC cultured in vitro and XZD exerts a certain protective effe ct on VEC injured by the serum of BSS rabbits.
