Role of apoptosis in the cell death of rat intestinal epithelium during the early stage of acute necrotizing pancreatitis
- VernacularTitle:细胞凋亡在急性坏死型胰腺炎早期肠黏膜上皮细胞死亡中的作用
- Author:
Xingpeng WANG
;
Bingxian WANG
;
Kai WU
- Publication Type:Journal Article
- Keywords:
Acute pancreatitis;
Intestinal mucosal barrier;
Apoptosis
- From:
Chinese Journal of Digestion
1996;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether apoptosis of intestinal epithelial cells occurs at the early stage of acute necrotizing pancreatitis (ANP) in rats. Methods Fourty eight Spraque Dawley rats were used. ANP model of rats was induced by retro injection of 5% sodium taurocholate into biliopancreatic duct. Laparotomized animals without induction of ANP (sham operation) served as controls. The distal segment of ileum specimens were harvested at 3 h, 6 h, 12 h and 24 h after operation and the apoptosis of intestinal epithelial cells was studied by DNA gel electrophoresis, FITC conjugated annexin V and propidium iodide (PI) staining cells analyzed by Flow Cytometry (FCM) and immunohistochemical procedures (TUNEL method). Results The DNA electrophoresis showed that typical “ladder” patterns appeared at all indicated time points in ANP group, while the DNA specimens from control group presented a single chromosomal lane, except the one at 12 h. Apoptotic percentage of detached intestinal epithelial cells assayed using Annexin V kit by FCM were (53.7?3.7)%, (27.6?6.0)%, (39.0?4.8)%, (29.0?11.3)% at 3 h, 6 h, 12 h and 24 h in control group and (50.3?11.3)%, (79.7?9.2)%, (47.8?17.3)%, (49.6?9.5)% in ANP group. There was a significant difference between two groups at 6 h, P
