Determination of lcariin in Bushenning Capsules by HPLC
- VernacularTitle:高效液相色谱法测定补肾宁胶囊中淫羊藿苷的含量
- Author:
Dong XU
;
Lili SUN
;
Shubin YANG
;
Jingyong SUN
- Publication Type:Journal Article
- Keywords:
Bushenning Capsules;
HPLC;
icariin;
determination
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a RP-HPLC method for the determination of icariin in Bushenning Capsules. Methods: The sample was prepared by water extraction-purfication through polyamide column. The determination was carried out on C 18 ODS column with mobile phase of acetonirile-water (23∶77), and detection wavelength at 270nm according to the external standard method.Results: The icariin sample size showed a good linear relationship at the range of 0.12-1.91ug and correlation coefficient was 0.9999. The average recovery of the added sample was 99.21%(n=5) and RSD was 1.79%. The RSD in a duplicate test was 2.18%.Conclusion: The method is simple, accurate, reproducibe and can be used for content determination of icariin of icariin of Bushenning Capsules.
