Study of combination therapy with antisense insulin like growth factor Ⅱ and MDP in the treatment of hepatocellular carcinoma
- VernacularTitle:反义胰岛素样生长因子Ⅱ与胞壁酰二肽联合抗肝癌实验研究
- Author:
Xiaoyan NING
;
Donghua YANG
;
Chong XU
- Publication Type:Journal Article
- Keywords:
Antisense;
Insulin like growth factor Ⅱ;
Muramyl dipeptide;
Liposomes;
Hepatocellular carcinoma
- From:
Chinese Journal of Digestion
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Effects of hepatocellular carcinoma of antisense insulin like growth factor Ⅱ (antisense IGF Ⅱ) combined with muramyl dipeptide (MDP) was studied. Methods A cDNA fragment of human IGF Ⅱ was synthesized and inserted into a eukaryotic expression vector of pcDNA 3 (antisense IGF Ⅱ ). Antisense IGF Ⅱ and MDP encapsulated with liposomes were introduced into HepG 2 human hepatocellular carcinoma cells and were injected into subcutaneous tumors generated in nude mice. The growth of HepG 2 cells transfected with antisense IGF Ⅱ and MDP, and the subcutaneous tumors injected with antisense IGF Ⅱ and MDP were observed. Results The growth of HepG 2 cells was blocked completely, and the subcutaneous tumors disappeared in two of five nude mice. Conclusions The combination therapy of antisense IGF Ⅱ and MDP is effective in the treatment of hepatocellular carcinoma.
