Simultaneous Detection of Common Food-borne Bacterial Pathogens in Innocuous Poultry and Seafood by Multiplex PCR Assay
- VernacularTitle:多重PCR检测无公害畜禽肉和水产品中4种致病菌
- Author:
Xiaojuan YANG
- Publication Type:Journal Article
- Keywords:
Food-borne bacterial pathogens, Multiplex PCR, Poultry and seafood
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
To develop a multiplex PCR assay to detect Enterohemorrhagic Escherichia coli (EHEC), Vibrio parahaemolyticus (VP), Salmonella spp. and Listeria monocytogenes(LM) in innocuous poulthy and seafood simultaneously, four pairs of primers were designed from hemolysis gene (hlyAB) of EHEC, toxR gene of VP, invA gene of Salmonella spp. and iap gene of LM. They were detected by conventional PCR and multiplex PCR assay. DNA sequencing was used to examine PCR products. This multiplex PCR method was a highly specific, rapid and sensitive method which could simultaneously detect EHEC, VP, Salmonella spp. and LM. The detection level was 10~(3)cfu/mL.
