Gene transfer of CD_( 80)into a human ovarian cancer cell line and induction of cytotoxic T lymphocyte in vitro
- VernacularTitle:CD_(80)基因导入人卵巢癌细胞系及其体外诱导细胞毒T淋巴细胞的研究
- Author:
Baoxia CUI
;
Beihua KONG
;
Jie JIANG
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Antigens, CD 80;
Transfection;
T lymphocytes, cytotoxic
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the proliferation and cytotoxicity of cytotoxic T lymphocyte (CTL) induced by ovarian cancer cells transfected with a CD 80 gene containing retroviral vector Methods The ovarian cancer cell line TYK cells were transfected with retro virus vector PLXSN hCD 80 After geneticin (G418) selection, the CD 80 expression of the transfectants was confirmed by flow cytometry CTL was induced by co culture of CD 80 expressing TYK cells (TYK hCD 80 ) and peripheral blood mononuclear cells (PBMC) in the presence of anti CD 3 monoclonal antibody (McAb) Proliferation of PBMC was measured using 3?H Thymidine incorporation assay The lysis activity of CTL toward tumor cells was determined using methyl thiazolyl tetrazolium (MTT) assay Results After transfection and G418 selection, the CD 80 expression was proved by flow cytometry The highest rate of CD 80 expression was 84 9% The TYK cell line expressing high CD 80 was named TYK hCD 80 In the presence of anti CD 3 McAb,TYK hCD 80 significantly enhanced proliferation of PBMC than TYK cells ( 3H Thymidine incorporation, (40 604? 842) vs (8 000? 594) cpm ( P
