THE EFFECTS OF CHOLERAL TOXIN ON THE REGENERATIONOF THE RETINAL GANGLION CELLS IN GOLDEN HAMSTER
- VernacularTitle:霍乱毒素对金黄地鼠视网膜节细胞再生的作用
- Author:
Wen LI
;
Haibiao LI
- Publication Type:Journal Article
- Keywords:
Cholera toxin;
Sciatic nerve;
Retinal ganglion cells;
Axonal regeneration;
Golden hamster
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The present study was aimed at investigating the effects of cholera toxin(CTx) on promoting the regeneration of retinal ganglion cells(RGCs) in hamster retina. Methods After optic nerve (ON) transection, an autologus sciatic nerve (attached graft, AG) was removed and sutured to the proximal stump of the ON. CT X was injected or (and) a small segment of sciatic nerve (SN) inserted intravitrously. Animals were separated into 5 groups:regenerating control group(AG groups and solution groups); AG+CT X groups; AG+SN groups; AG+CT X+SN groups; Effect and Dosage groups; Animals in the former 4 groups were allowed to survive for 2-6 weeks respectively. The regenerated RGCs were labeled retrogradely by granular blue, and the changes in number of regenerating RGCs in each retina were observed under fluorescent microscope. Results The mean numbers of regenerating RGCs in AG+CT X groups were increased and significantly higher than those in AG groups and solution groups at each time point( P
