OBSERVATION OF THE DISTRIBUTION OF ?-AMINOBUTYRIC ACID INTHE VISUAL CORTEX OF THE RECONSTRUCTED RCS RAT′S RETINABY TRANSPLANTING PURE PHOTORECEPTOR CELLS
- VernacularTitle:纯视细胞移植术后RCS鼠视皮质内?-氨基丁酸的分布
- Author:
Feng PAN
;
Wei WANG
;
Li SHEN
;
Le YANG
- Publication Type:Journal Article
- Keywords:
aminobutyrate(GABA);
Retina transplantation
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective We observe the ?-aminobutyric acid(GABA)′s variation in the visual cortex of Royal College of Surgeons Rat(RCS rat)′s retina following the photoreceptor cell transplantation. Method The healthy Wistar rats′photoreceptor cells were grafted into the sub-retinal space of the left eye of the RCS rat by outer-path way.Two weeks later,host animals were sacrificed.Both sides of visual cortex were processed for light microscopic analysis.So did the 74 days Wistar rats and 74 days RCS rats.Then immunocytochemistry was used to determine the localization of the amino acid neurotransmitters(?-aminobutyrate,GABA).Results After two weeks of transplantation,the transplanted photoreceptor cells survived.On the opposite side of the visula cortex in the grafted RCS rats,GABA-like immunoreactivity was intensely stained.But the same side of the visual cortex in the grafted RCS rats,GABA-like immunoreactivity could not be observed.Conclusion These results suggest that we can rebuild the visual pathway by transplanting the photoreceptor cells.In the visual cortex of the grafted RCS rats, GABA- like immunoreactivity may indicate that the visual cortex had reflection to the stimulation after photoreceptor cells had been grafted.
