Regulation of the telomerase activity by recombinant human tumor necrosis factor in vitro
- VernacularTitle:重组人肿瘤坏死因子调节端粒酶活性的体外研究
- Author:
Weifen XIE
;
Yong LIN
;
Xingrong ZHANG
- Publication Type:Journal Article
- Keywords:
Recombinant human tumor necrosis factor (rhTNF);
Human telomerase reverse transcriptase (hTERT);
Gene regulation
- From:
Chinese Journal of Digestion
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of recombinant human tumor necrosis factor (rhTNF) on telomerase activity in hepatoma cell line HepG2 cells and HepG1 6 cells. Methods TRAP ELISA method was used to determine the telomerase activity in HepG2 and HepG1 6 cells which were treated by different concentrations of rhTNF; plasmid which had been inserted 800 bp of the hTERT promoter was transiently transfected into HepG2 cells by Lipofect, and different concentrations of rhTNF were added into culture media 2 hours later, then the activity of the hTERT promoter was tested 48 hours after transfection. Results The telomerase activity of HepG2 was suppressed by rhTNF in a dose dependent manner. The expression of hTERT promoter was inhibited linearly with the dose of rhTNF within the range of 10～1 000 IU/ml. Conclusions Above results suggest that the anti tumor activity of rhTNF may attribute to its inhibitory effect on hTERT promoter expression.
