Effects of interferon-? gene-modified hepatocytes on implanted liver carcinoma
- VernacularTitle:?-干扰素基因修饰肝细胞对移植性肝肿瘤治疗的实验研究
- Author:
Jianhang LENG
;
Lihuang ZHANG
;
Hangping YAO
- Publication Type:Journal Article
- Keywords:
Interferon ?;
Gene therapy;
Implanted liver carcinoma
- From:
Chinese Journal of Digestion
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effects of intrasplenically transplanted interferon ? (IFN ?) gene modified hepatocytes on murine implanted liver carcinoma. Methods Murine fetal hepatocytes (BNL?CL2) were transfected with recombinant adenovirus expressing IFN ?. Two cell lines BNL?Lac Z and BNL?CL2 were taken as control. One week after intrasplenically injected C26 cells(colon carcinoma line), sixty tumor bearing syngeneic mice were intrasplenically transplanted IFN ? gene modified hepatocytes and were divided into treated group (BNL?IFN ?) and two control groups (BNL?Lac Z and BNL?CL2), two weeks later, levels of IFN ?, tumor necrosis factor alpha(TNF ?) and nitric oxide(NO) in the serum of liver implanted carcinoma bearing mice were assayed, the cytotoxicity of murine splenic cytotoxic T lymphocyte (CTL) was measured. Mophology of hepatic tumors were studied. The therapeutic effects on the mice with the implanted liver carcinoma were also evaluated. Results In treated group (compared with control groups), the levels of IFN ?, TNF ? and NO in the serum increased significantly ( P
